Tfeb-s211a

Author: qvns

August undefined, 2024

Web17 Jul 2024 · Under un-treated condition, WT TFEB showed ~30% nuclear localization, TFEB-S142A showed ~45% nuclear localization, while TFEB-S211A showed ~70% nuclear … Web6 Dec 2024 · However, expression of TFEB-S211A and TFE3-S321A led to a near uniform pattern of p53 expression, predominantly localized in the nuclei of HeLa cells (Figure 5C). Given our data in RAW264.7 cells suggesting that loss of TFE3 and TFEB decreases p53 half-life, we performed a complimentary cycloheximide chase experiment to test if …

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Web11 Feb 2013 · The increased ability of the TFEB-S211A mutant to associate with lysosomes when compared with TFEB-WT suggests that the binding of 14-3-3 to TFEB may mask … Web2 Feb 2024 · To test whether TFEB or TFE3 can induce GPNMB expression, we expressed constitutively nuclear mutants of TFEB (TFEB-S211A) or TFE3 (TFE3-S321A) in T47D and … patricia briggs silence fallen

STUB1 regulates TFEB-induced autophagy–lysosome …

Total RNA was extracted from cellular lysates using the RNeasy Mini kit (Cat# 74104, Qiagen) according to the manufacturer’s instructions. cDNA was synthesized by reverse transcription of total RNA (500 ng per sample) using QuantiTect Reverse Transcription kit (Cat# 205311, Qiagen). The generated cDNA was … See more Cells were cultured in the following media: HeLa (ATCC) in MEM (Cat# ECB2071L, Euroclone); HEK293T (ATCC) in DMEM high glucose (Cat# ECM0728L, Euroclone); ARPE19 (ATCC) in … See more Reagents used in this study were obtained from the following sources: Antibodies to Phospho-p70 S6 Kinase (Thr389) (1A5) (Cat# 9206) (1:1000), … See more For experiments involving amino acid starvation, cell culture plates were rinsed twice with PBS and incubated in amino acid-free RPMI (Cat# R9010-01, USBiological) supplemented with 10% dialyzed FBS for 60 … See more The plasmid-encoding ΔNLS-TFEB-GFP was a kind gift of Shawn Ferguson (Yale School of Medicine, CT, USA). TFEB S138A-GFP was a kind gift of Chonglin Yang (Chinese … See more Web5 Mar 2024 · To further test the role of TFEB in vivo, the authors performed mouse xenograft experiments using AML cells with dox-inducible TFEB S211A. Consistent with ex vivo … WebThe master regulator of lysosome biogenesis, TFEB, is regulated by MTORC1 through phosphorylation at S211, and a S211A mutation increases nuclear localization. However, … patricia briggs dragon blood

TFEB and TFE3 are novel components of the integrated stress response …

Addgene: pCIP-Flag-TfebAA

WebTFEB-mediated endocytosis drives assembly of the MTORC1-containing nutrient sensing complex through the formation of endosomes that carry the associated proteins RRAGD, the amino acid transporter SLC38A9, and activate AKT/protein kinase B (AKT p-T308). Web25 Jan 2016 · TFEB and TFE3 are novel components of the integrated stress response. José A Martina Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. Author profile Search articles by ORCID 0000-0001-5814-8807 Martina JA1, Heba I Diab patricia brignoneWeb12 Apr 2024 · PLX4720 ablated TFEB S142 phosphorylation in A375 cells (Fig. 3a ). An in vitro kinase assay using bacterially purified proteins showed that active ERK directly … patricia brightful passing

"Web17 Aug 2024 · In particular, TFEB S142A showed impaired phosphorylation of S142 and S138; TFEB S138A showed impaired phosphorylation of S138, but normal S142 phosphorylation; importantly, in TFEB S211A, which still retained the ability to be exported from the nucleus, S142 and S138 were normally phosphorylated (Fig. 4c). These data … " - Tfeb-s211a

Tfeb-s211a

mTOR-dependent phosphorylation controls TFEB nuclear export

Web1 Apr 2024 · To test whether Nanog was a potential target gene of TFEB, a constitutively active form of TFEB (TFEB-AA, mutated S142/S211 to S142A/S211A) and a reporter … WebNational Center for Biotechnology Information

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WebFor mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb. Depositing Lab. Reuben Shaw. Publication. Young et al Genes Dev. 2016 Mar … WebMYC-tagged plasmids (WT, wild-type TFEB; S211A TFEB mutant or EV, empty vector), lysed, and treated or not with lambda phosphatase (“λ”) before MYC immunoprecip-itation (IP) …

Web22 Mar 2024 · Transcription factor EB (TFEB) is a basic helix–loop–helix–leucine–zipper transcription factor, which is considered the main regulator of lysosomal biogenesis and autophagy, and mediates various physiological processes, including immune response, neurodegenerative diseases, and metabolic diseases ( 12 – 15 ). Web24 Nov 2024 · We demonstrate that TFEB is a direct substrate of PTEN phosphatase and PTEN deficiency increases exosome secretion in cholangiocarcinoma by reducing lysosome biogenesis, which further promotes tumor metastasis.

Web11 Dec 2024 · Previous studies showed TFEB is a nucleocytoplasmic shuttling protein and phosphorylated TFEB is captured by YWHA thus remains in the cytosol. Given that TFEB could translocate into the nucleus under numerous of cellular stress, we wondered whether oxidative stress induced by ROS could affect nucleocytoplasmic shuttling of TFEB. Web28 Jul 2024 · Flag-tagged S142A/S211A-TFEB mutant was cotransfected, in HEK293 cells, with a GFP-tagged TFEB construct (wild-type, S142A/S211A, or S142D/S211D). Co-immunoprecipitation analysis, using Flag antibody, …

Web1 Jul 2016 · The transcription factor EB (TFEB) plays a pivotal role in the regulation of basic cellular processes, such as lysosomal biogenesis and autophagy. The subcellular …

WebTFEB acts as a link between autophagic process and lysosome biology [160], interacting with mTORC1 complex but not with mTORC2 controlling the lysosomal localization and function of mTORC1 [161].... patricia brinsonWebTFEB is a transcription factor that regulates both autophagy and lysosomal biogenesis via rapid translocation to the nucleus from the cytosol and lysosomes upon starvation (4, 12– 14). Correspondingly, overexpression of TFEB has been shown to affect the expression of a unique set of genes that are related to lysosome function and autophagy (5). patricia bright financial plannerWeb24 Mar 2024 · On day two, levels of TFEB (S211A)-GFP and TFEB targets were down to Dark levels, however the AT8 + and AT180 + p-Tau levels seem to have gradually raised but still remained significantly lower than their starting levels (Fig 5E). Taken together, for the first time, these results suggest that light-induced, optogenetic-based expression of TFEB can … patricia briosoWeb24 Mar 2024 · TFEB, we tested S211A mutation in TFEB, which was previously shown to prevent phosphory- lation by mTORC1 [ 34 ] thereby facilitates TFEB’s nuclear entry. Given that the transcriptional patricia brinkman omaha neWeb5 Jan 2024 · Specifically, TFEB S211A-GFP, unlike wild-type TFEB-GFP, diffusely localized throughout the cytosol and nucleus (Fig. 1E, F). Figure 1. Torin1 enhances TFEB S211A … patricia britt hartsville scWeb24 Mar 2024 · On day two, levels of TFEB (S211A)-GFP and TFEB targets were down to Dark levels, however the AT8 + and AT180 + p-Tau levels seem to have gradually raised but still remained significantly lower than their starting levels . Taken together, for the first time, these results suggest that light-induced, optogenetic-based expression of TFEB can … patricia britschgiWeb26 Jan 2016 · In addition, over-expression of TFEB-S211A further augmented the stress-induced increase in ASNS, CAT1, and xCT levels (Fig 6A–D). Notably, depletion of ATF4 … patricia brolin ribi