WebSignificantly, after boiling the UV-irradiated nuclear extract, only the 50-kD photoadduct was immunoprecipitated (Fig. 2B, lane 4), demonstrating that this adduct indeed contains the Lsm11 protein. The N terminus of Lsm 11 is essentialfor histone RNA 3′ end processing. To investigate whether Lsm11 plays a role in histone RNA processing, we ... WebThe photoadduct was characterized by various physicochemical methods. Results: Photoaddition of arginine to 200 bp DNA rendered the nucleic acid conformer thermodynamically more stable than the native form. After systematic characterization of the photoadduct, it was used as an antigen for the generation of antibodies in …
Formation of an Adenine-Thymine Photoadduct in the ... - Science
WebAug 21, 2013 · This photoadduct after milling for 24 h in G5 planetary ball mill led to the formation of nanophotoadduct, which is then used as dopant in PTh to form nanocomposite via chemical oxidative polymerization. Crystallite size and strain analysis from XRD using different methods confirms nano dimension of photoadduct and revealed good correlation. WebJun 11, 2015 · Nanocomposite of polythiophene (PTh) with the photoadduct of sodium pentacyanonitrosylferrate(II) dihydrate and hexamine is synthesized by In-situ polymerization technique. Photoadduct was obtained by photoirradiation followed by substitution with hexamine ligand using Osram UV–visible photo lamp which was proved … siadh water retention
An optogenetic gene expression system with rapid activation and ...
WebAug 26, 2012 · The present paper involves the synthesis of polyaniline (PANI) composite with photoadduct of potassium hexacyanoferrate (II) involving hexamine as a ligand and cobalt chloride as a complexing agent via in situ oxidative polymerization by ammonium persulphate. The photoadduct has been synthesized by photoirradiation followed by … WebWe report the first structure determination of a covalent photoadduct between a RuII-tap complex and a tryptophan-containing peptide (AlaTrpAla) by mass spectrometry and NMR spectroscopy. RuII-tap complexes could thus be exploited as photodamaging agents of Trp-containing polypeptides or proteins. WebARTICLE Detection of the thietane precursor in the UVA formation of the DNA 6-4 photoadduct Luis A. Ortiz-Rodríguez 1, Christian Reichardt1,3, Sean J. Hoehn 1,3, Steffen Jockusch 2 & Carlos E ... siadh urine specific gravity