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Phis2.1

WebbThe target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein and the target sequence stimulates … WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

Phis2 1 TaKaRa Bioz

Webb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. Webb6 feb. 2024 · Europe PMC is an archive of life sciences journal literature. gps holerite online https://tri-countyplgandht.com

pHis2载体质粒图谱、序列、价格、抗性、大小等基本信息_生物风 …

Webb24 mars 2024 · In case of 4CRM1-pHis2.1, 4CRM1mut2-pHis2.1, and 4CRM1mut5-pHis2.1, 50 mM 3-amino-1,2,4-triazole (3-AT) was sufficient to suppress background growth of yeast colonies. DNA from the TF-only prey library in pDEST_GAD424 (Mitsuda et al. 2010) was transformed into Y1HGold containing 4CRM1-pHis2.1. Webb15 okt. 2024 · For the protein–DNA-binding experiment, an oligonucleotide sequence containing triple tandem copies of the W-box and mW-box was synthesised, annealed, and cloned into the pHIS2.1 vector forming W-box- and mW-box-specific reporter vectors. http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf gps holder for motorcycle

pBluescript II SK(+) Sequence and Map - SnapGene

Category:Disruption of the bHLH transcription factor Abnormal …

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Phis2.1

VvWRKY30, a grape WRKY transcription factor, plays a …

Webb27 mars 2015 · In case of pHis2.1-Seq15 containing 4CRM-DJ1E, 50 m m 3-AT was sufficient to suppress background growth. As prey to select for TFs that bind to the …

Phis2.1

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Webb17 juni 2024 · Expression pattern analysis of OsbZIP62 under abiotic stresses and hormones. a, Relative expression level of OsbZIP62 under various stresses, including PEG6000 (15%), cold (4 °C), heat (42 °C), NaCl (150 mM), H 2 O 2 (1%) and ABA (100 μM). b, Main stress-related cis-acting elements in the OsbZIP62 promoter region.c, Relative … WebbBait Vector Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > bait vector phis2. bait vector phis2 TaKaRa is a verified supplier TaKaRa ...

WebbPlasmid pHIS2-hsSTAM1 (1-143) from Dr. James Hurley's lab contains the insert phis2- STAM1(1-143) and is published in Structure. 2009 Mar 11. 17(3):406-16. This plasmid is … Webb16 feb. 2024 · To explore the involvement of bZIP genes in flower senescence, one up-regulated transcript encoding a putative ocs element binding factor, namely PhOBF1 (SGN accession no. Peaxi162Scf00285g00011), was identified during petunia flower senescence through transcriptome sequencing analysis [ 36 ].

WebbpHIS2-hsSTAM1 (1-143) Sequences pHIS2-hsSTAM1 (1-143) Sequences (2) Addgene Sequences: Partial (1) Depositing Scientist Sequences: Partial (1) Partial Sequences … Webb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (Camellia sinensis L.) CBF gene (CsCBF) was still seldom reported. Here, functional …

Webb26 mars 2024 · The promoter sequence (1800 bp) of MdMATE8 (MDP0000175055) was inserted into the pHIS2.1 vector (Clontech, Palo Alto, CA, United States), which contained …

WebbPhis2 1 Bait Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2 1 bait plasmid. phis2 1 bait plasmid TaKaRa is a ... gps holder for motorcycle handlebarWebb1 sep. 2024 · 1-Methylcyclopropene counteracts ethylene promotion of fruit softening and roles of MiERF2/8 and MiPG in postharvest mangoes. ... and pHIS2-MiPG were cultivat ed on SD/-T r p/-His for 3 days, and . chili dog sauce with beans recipeWebb1 dec. 2024 · According to the hereditary mode of sterility genes, this vital agronomic trait can be further divided into cytoplasmic male sterility (CMS) and genic male sterility … gps holder on dashboardWebb14 jan. 2015 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ... chili down lyricsWebb5 feb. 2024 · Either pGADT7-RrMYB18 or pGADT7-AtMYB46 was used to co-transform yeast strain Y187 with either the pHIS2.1-P RrCESAs or pHIS2.1-P AtCYCs reporter plasmids, respectively. DNA–protein interactions were quantified using the Matchmaker one-hybrid system (Clontech, now TaKaRa) according to the manufacturer’s instructions … chili downshiftologyWebb+ 5 µg pHis2.1 20 µl Note: To denature carrier DNA, heat to 95–100°C for 5 min, then cool rapidly in an ice bath. NOTE NOTE Protocol No. PT3529-1 Version No. PR7Z2445 32 3. Add competent cells and gently mix. 4. Add PEG/LiAc and gently mix. 5. Incubate at 30°C . NOTE: Mix cells every 10–15 min. www.clontech.com 50 µl 500 µl 30 min 600 µl 2.5 ml chili dragon burnaby menuWebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … gpshome login