Lamina joint inclination assays
WebOct 3, 2024 · Therefore, lamina joint inclination assay and RNA sequencing (RNA-Seq) analysis were performed here. ABA antagonizes the promotive effect of BR on leaf angle. Hundreds of genes responsive to both hormones that are involved in leaf-angle determination were identified by RNA-Seq and the expression of a gene subset was … WebNational Center for Biotechnology Information
Lamina joint inclination assays
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WebMar 9, 2024 · Lamina joint inclination assays showed that the lamina joint bending angles of the T 1 progenies from the uORF OsBRI1 (−99, 28 aa)#14 were smaller than … WebFeb 5, 2024 · For lamina joint inclination assay, sterilized rice seeds were grown in the dark at 30 °C for 9 days. We obtained equally sized samples by excising approximately 2 …
WebA rice lamina inclination test that is simple and specific for brassinosteroids was used as a micro-quantitative bioassay for brassinolide 1 and its 6-keto congener, castasterone 2, in the concentration range of 5 x 10- 5 Jlg/ml to 5 x 10- 3 Jlg/ml, when uniform seedlings of the rice WebNov 29, 2024 · We functionally characterize a novel rice SPOC domain-containing protein leaf inclination 3 (LC3), whose deficiency (lc3 mutant) presents enhanced leaf angle due to the excessive cell elongation at adaxial side of lamina joint, and demonstrate that LC3 controls leaf inclination by regulating auxin signaling through interacting with LIP1, a HIT ...
WebDec 5, 1998 · It has been found that pretreatment with 0.1 and 1 μg of IAA before application of brassinolide increased activity by 10- and 100-fold, respectively, in the rice lamina … WebAug 1, 2009 · The lamina joint bending assay is established as an indicator to evaluate the effects of plant hormones, herbi- cides and light on rice leaf bending, which is thought to affect crop yields (...
WebJan 29, 2016 · For the lamina inclination assays, 1 µl of ethanol containing a series of concentrations of BRs, IAA or Brz was spotted onto the tips of the lamina of 5- or 6-day-old seedlings. C-22-hydroxylated and 6-deoxo brassinosteroids were identical to those described by Nakamura et al. (2009).
WebMar 8, 2024 · The leaf bending caused by extracts from Distylium racemosum in the rice lamina inclination assay is one of the earliest reports of brassinolide (1) activity in plants . In this assay, 1 caused dose-dependent swelling of the adaxial cells in the joint between the leaf blade and sheath of etiolated rice seedlings. total number of lone pairs in ncl3WebA rice lamina inclination test that is simple and specific for brassinosteroids was used as a micro-quantitative bioassay for brassinolide 1 and its 6-keto congener, castasterone 2, in … total number of lone pairs in scl2WebBrassinosteroid (BR)-induced rice (Oriza sativa L.) lamina joint (RLJ) inclination and its relationship to indole-3-acetic acid (IAA) and ethylene were investigated using BR … post op quality improvement inititativeWebJan 27, 2024 · Lamina joint inclination test To test the sensitivity of ZH11, rel1, rip2, RIP2 -OE, and rel1rip2 to 24-epibrassinolide (24-epiBL) in a lamina joint inclination experiment, ethanol containing 0, 10, 100, or 1000 ng of 24-epiBL was applied to the lamina joint of the first leaf of rice seedlings. post op rash around incisionWebMay 12, 2024 · Interestingly, ELT1 is preferentially expressed at the lamina joint (Figure 1E) and tiller buds (Figure 1F), and transcribed throughout the lamina joint development (Supplementary information, Figure S5A and S5B), which is consistent with the altered leaf inclination and tiller numbers in mutants. total number of lone pair in xef2WebMar 18, 2024 · Scanning electron microscopy revealed that the expansion of cell size in the adaxial side of the lamina joint was responsible for the increase in leaf angle in osbhlh079-D. The expression of... post op pulmonary insufficiency criteriaWebDec 1, 2024 · OsIAA6 interacts with OsARF1 to suppress auxin signaling and to promote cell elongation at the adaxial side of the lamina joint, leading to enlarged leaf inclination. OsBZR1 binds directly to OsIAA6 promoter and OsIAA6 mediates the synergistic effects of BR and auxin on lamina joint development. OsIAA6 may interact with other OsARFs to … post op recovery home