Webdetritylation solution with ESI -MS (Method B). Specifically, a portion of the last batch of detritylation solution was concentrated to dryness and dissolved in methanol (e.g. 2 … WebTraditionally dilute solutions of trichloroacetic acid (TCA, pK a 0.8) or dichloroacetic acid (DCA, p Ka 1.5) in dichloromethane (DCM) were used as detritylation reagent for ON synthesis. 125b, 132 Later, it was recognized that a high concentration of a weak acid (15% DCA in DCM) is more effective at driving detritylation toward completion than ...

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WebJan 7, 2009 · Addition of small amounts of water to the reaction medium reduces the detritylation rate, presumably through its effect on the solution acidity. All observations are compatible with detritylation occurring through a concerted general acid-catalysed mechanism rather than a stepwise A1 process. WebFigure 2. The detritylation mechanism. The 5'-DMT protecting group is removed by TCA (trichloroacetic acid) in the solvent dichloromethane (too concentrated a solution of TCA or too long of a detritylation … phoenix os 32 bits 2021

Tritylation - an overview ScienceDirect Topics

Web1. Charge the synthesizer with solvents and detritylation solution needed for the steps shown in Table 3.4.1. These can usually be kept on the synthesizer until they are consumed. 2. Dissolve the protected nucleoside 3 -H-phosphonates (triethylammonium salts) in pyridine and evaporate the solvent (two times) under reduced pressure in a rotary ... WebAbstract. The kinetics and mechanism of the deprotection (detritylation) of 5′- O - (4,4′-dimethoxytrityl)-2′-deoxythymidine nucleoside catalysed by dichloroacetic acid to give … WebThe rate of detritylation is generally faster than the rate of depurination and to minimise the amount of depurination, detritylation is often performed as quickly as possible. 3,6 To … how do you find the radian of a degree