WebFluorescence staining. 1. The antibody coupled with fluorescence dyes should be preserved at 4℃ in dark. 2. Blocking reagents such as 0.5% BSA and 1% fetal bovine serum should be used to block non-specific binding sites. 3. The sample stained by fluorescence dyes should be kept away from lights to make sure its stability. WebDQ Red BSA is a fluorogenic substrate for proteases. A strong fluorescence quenching effect is observed when proteins are heavily labeled with BODIPY dyes. Upon hydrolysis …

Stain Buffer (BSA) - BD Biosciences

WebWash the cells twice in cold Pharmingen Stain Buffer (BSA) and pellet the cells by centrifugation (e.g., 300 x g at 4°C). Resuspend the cell. pellet with cold Pharmingen Stain Buffer (BSA) to a final concentration of 2 x 10e7 cells/ml. 3. Distribute 50 µl aliquots of the cell suspension (10e6 cells) to either tubes or the round-bottomed wells ... Web产品说明. The Human Monocyte STING Activation Flow Cytometry Panel includes antibodies targeting phospho-STING (Ser366) and phenotyping markers to enable observation of STING activation in human monocyte populations among peripheral blood mononuclear cells (PBMCs). CD45 is a pan leukocyte marker. Monocytes and dendritic … therapeutic riding ireland

FACS Buffer: Why include FBS? ResearchGate

WebSix α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes … WebGeneral procedure for flow cytometry using a primary antibody and conjugated secondary antibody. Print this indirect flow cytometry protocol. ... if necessary, should be made in … WebThe Human Monocyte STING Activation Flow Cytometry Panel includes antibodies targeting phospho-STING (Ser366) and phenotyping markers to enable observation of STING activation in human monocyte populations among peripheral blood mononuclear cells (PBMCs). CD45 is a pan leukocyte marker. therapeutic robotic pets